gst smad3 mh2 Search Results


gst smad3 mh2  (Addgene inc)
85
Addgene inc gst smad3 mh2
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Gst Smad3 Mh2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/pcDNA3-HA-CUL1+delta+C22+(Plasmid+%2319942)/pm22782721-79-0-10
Average 85 stars, based on 1 article reviews
gst smad3 mh2 - by Bioz Stars, 2026-09
85/100 stars
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linker mh2 146 425 domains  (Addgene inc)
93
Addgene inc linker mh2 146 425 domains
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Linker Mh2 146 425 Domains, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/pAc5%2E1B-lambdaN-HA-DmAGO1-PAZ10-siRNAres_G+(Plasmid+%23146425)/pm22782721-79-4-10
Average 93 stars, based on 1 article reviews
linker mh2 146 425 domains - by Bioz Stars, 2026-09
93/100 stars
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mh2 gst fusion proteins  (Danaher Inc)
96
Danaher Inc mh2 gst fusion proteins
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Mh2 Gst Fusion Proteins, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/Fusion/10__1074_slash_jbc__m304856200-99-4-26
Average 96 stars, based on 1 article reviews
mh2 gst fusion proteins - by Bioz Stars, 2026-09
96/100 stars
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glutathione sepharose 4b  (Danaher Inc)
99
Danaher Inc glutathione sepharose 4b
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Glutathione Sepharose 4b, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/Glutathione+Sepharose+4B/10__1074_slash_jbc__m106826200-111-39-41
Average 99 stars, based on 1 article reviews
glutathione sepharose 4b - by Bioz Stars, 2026-09
99/100 stars
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pgex 4t2  (Danaher Inc)
95
Danaher Inc pgex 4t2
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Pgex 4t2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/PGEX-4T-2/10__1074_slash_jbc__m304856200-99-25-26
Average 95 stars, based on 1 article reviews
pgex 4t2 - by Bioz Stars, 2026-09
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pgem-3zf  (Promega)
90
Promega pgem-3zf
Fig. 1. Endogenous <t>Smad3</t> and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), <t>MH2</t> (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.
Pgem 3zf, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+smad3+mh2/pgem+t+easy/pm17429065-27-20-22
Average 90 stars, based on 1 article reviews
pgem-3zf - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Fig. 1. Endogenous Smad3 and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), MH2 (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.

Journal: Development (Cambridge, England)

Article Title: Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.

doi: 10.1242/dev.078345

Figure Lengend Snippet: Fig. 1. Endogenous Smad3 and JMJD3 interact in NSCs. (A)Size-exclusion chromatography of NSC lysate showing co-elution of Smad3P and JMJD3, and the presence of Smad3 in the lower weight fractions. (B)Co-IP of mouse NSCs lysate using anti-Smad3P antibody or unrelated IgGs in the presence or absence of TGFfor 30 minutes. (C)Upper panel shows schematic representation of GST-Smad3 fragments: full length (FL), MH1 (1-155 aa), MH2 (199-425 aa) and linker domains that also contains MH2 (146-425 aa). Pull-down assay using GST-Smad3 fusion proteins and 293t cell extracts overexpressing Myc-JMJD3. Ponceau staining of GST-Smad3 proteins (lower panel). (D)Immunoblot from control knockdown (C KD) and JMJD3 knockdown (JMJD3 KD) cell extracts using the indicated antibodies. (E)Immunoblot showing Nestin expression prior to and after TGFtreatment for the indicated times in C KD and JMJD3 KD cells. Nestin levels (relative to Actin) were quantified by using the Image J software (graph on the right). Input (In) corresponds to 1% of the protein present in the whole-cell extract.

Article Snippet: GST-Smad3 MH2 (199-425) and Linker-MH2 (146-425) domains were acquired from Addgene.

Techniques: Size-exclusion Chromatography, Co-Elution Assay, Co-Immunoprecipitation Assay, Pull Down Assay, Staining, Western Blot, Control, Knockdown, Expressing, Software